Anti-Lamin B1/LMNB1 Antibody (Clone#DC-12)

Rabbit Monoclonal Antibody

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筛选器： All WB IHC ICC/IF IP

BM3891

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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all(9)
Western blot analysis of anti-Lamin B1/LMNB1 antibody (BM3891). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Lamin B1/LMNB1 antigen affinity purified monoclonal antibody (BM3891) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Lamin B1/LMNB1 at approximately 72 kDa. The expected band size for Lamin B1/LMNB1 is at 67 kDa.
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Immunohistochemical analysis of paraffin-embedded Rat ovary, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin's lymphoma, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody at 1:100 dilution.
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mmunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded rat liver, using Lamin B1 Antibody.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis of HepG2 cells, using Lamin B1 Antibody.
all(9)
all(9)
Western blot analysis of anti-Lamin B1/LMNB1 antibody (BM3891). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Lamin B1/LMNB1 antigen affinity purified monoclonal antibody (BM3891) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Lamin B1/LMNB1 at approximately 72 kDa. The expected band size for Lamin B1/LMNB1 is at 67 kDa.
all(9)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Lamin B1/LMNB1 Antibody (Clone#DC-12)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ADLD; lamin B1; LMN; LMN2; LMNB; LMNB1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP

基因名称

LMNB1

克隆号

DC-12

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Lamin B1

计算分子量

66 kDa

实际分子量

72 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

Uniprot ID

P20700

文献引用格式

Lamin B1/LMNB1 Antibody (Clone#DC-12) (Boster Biological Technology, Wuhan, China. Catalog#BM3891)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Lamin-B1

实验方案>

实验步骤

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