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Anti-LEPR (长亚型) Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

BA1234

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of LEPR (长亚型) using anti-LEPR (长亚型) antibody (BA1234). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat cardiac muscle tissue lysates,
    Lane 2: human SMMC whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LEPR (长亚型) antigen affinity purified polyclonal antibody (BA1234) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LEPR (长亚型) at approximately 132 kDa. The expected band size for LEPR (长亚型) is at 132 kDa.

    all(3)
  • IHC analysis of LEPR (长亚型) using anti-LEPR (长亚型) antibody (BA1234).
    LEPR (长亚型) was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-LEPR (长亚型) Antibody (BA1234) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(3)
  • IHC analysis of LEPR using anti-LEPR antibody (BA1234).
    LEPR was detected in a paraffin-embedded section of human kidney tissue. The tissue section was incubated with rabbit anti-LEPR Antibody (BA1234) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(3)

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产品名称
Anti-LEPR (长亚型) Antibody
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
CD295; DB; HuB219; LEP R; LEPR; leptin receptor; OB R; OB receptor; OBR
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC
基因名称
LEPR
宿主
Rabbit
抗体亚型
IgG
免疫原
Polypeptide
计算分子量
132 kDa
实际分子量
132 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).
Uniprot ID
P48357  
文献引用格式
LEPR (长亚型) Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1234)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
Leptin receptor
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-LEPR (长亚型) Antibody

    筛选器: All WB IHC

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