Anti-PI3K p85 alpha/PIK3R1 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB

BA1352-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Figure 1. Western blot analysis of PI3K p85 alpha/PIK3R1 using anti-PI3K p85 alpha/PIK3R1 antibody (BA1352-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse HEPA1-6 whole cell lysates,
Lane 6: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PI3K p85 alpha/PIK3R1 antigen affinity purified polyclonal antibody (BA1352-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PI3K p85 alpha/PIK3R1 at approximately 85 kDa. The expected band size for PI3K p85 alpha/PIK3R1 is at 84 kDa.
all(1)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-PI3K p85 alpha/PIK3R1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

GRB1; p85; p85 ALPHA; PI 3 Kinase p85 alpha; PI 3-kinase p85α; PI3 Kinase p85 Alpha; PI3 kinase subunit p85 alpha; PI3K p85(alpha); PI3K regulatory subunit alpha; PIK3R1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

PIK3R1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human PI 3 Kinase p85 alpha, identical to the related rat and mouse sequences.

计算分子量

84 kDa

实际分子量

85 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Phosphatidylinositol 3-kinase regulatory subunit alpha is an enzyme that in humans is encoded by the PIK3R1 gene. Its gene is mapped to 5q13. the bovine PI3K p85 subunit consists of 2 closely related proteins, p85-alpha and p85-beta. They cloned cDNAs encoding both p85 subunits, each of which is a 724-amino acid polypeptide. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.

Uniprot ID

P27986

文献引用格式

PI3K p85 alpha/PIK3R1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1352-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

phosphoinositide-3-kinase, regulatory subunit 1(alpha)

蛋白名全称

Phosphatidylinositol 3-kinase regulatory subunit alpha(PI3-kinase regulatory subunit alpha/PI3K regulatory subunit alpha/PtdIns-3-kinase regulatory subunit alpha)

实验方案>

实验步骤

引用文献(0)>

清除