Anti-PI3K p85 alpha/PIK3R1 Antibody (Clone#17P38)

Rabbit monoclonal antibody

说明书一键复制产品信息

筛选器： All WB

BM5187

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Figure 1. Western blot analysis of anti-PIK3R1 antibody (BM5187). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PIK3R1 antigen affinity purified monoclonal antibody (BM5187) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PIK3R1 at approximately 85 kDa. The expected band size for PIK3R1 is at 84 kDa.
all(1)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-PI3K p85 alpha/PIK3R1 Antibody (Clone#17P38)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

GRB1; p85; p85 ALPHA; PI 3 Kinase p85 alpha; PI 3-kinase p85α; PI3 Kinase p85 Alpha; PI3 kinase subunit p85 alpha; PI3K p85(alpha); PI3K regulatory subunit alpha; PIK3R1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

基因名称

PIK3R1

克隆号

17P38

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human PI3 Kinase p85 alpha

计算分子量

84 kDa

实际分子量

85 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Uniprot ID

P27986

文献引用格式

PI3K p85 alpha/PIK3R1 Antibody (Clone#17P38) (Boster Biological Technology, Wuhan, China. Catalog#BM5187)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

实验方案>

实验步骤

引用文献(0)>

清除