Anti-Connexin 43/GJA1 Antibody

筛选器： All WB IHC IF

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  Western blot analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (BA1727). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human placenta tissue lysates,
  Lane 2: rat brain tissue lysates,
  Lane 3: rat heart tissue lysates,
  Lane 4: mouse brain tissue lysates,
  Lane 5: mouse heart tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Connexin 43/GJA1 antigen affinity purified polyclonal antibody (BA1727) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Connexin 43/GJA1 at approximately 43 kDa. The expected band size for Connexin 43/GJA1 is at 43 kDa.

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  IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (BA1727).
  Connexin 43/GJA1 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Connexin 43/GJA1 Antibody (BA1727) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IF analysis of Connexin 43/GJA1 using anti- Connexin 43/GJA1 antibody (BA1727).
  Connexin 43/GJA1 was detected in paraffin-embedded section of rat heart tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti- Connexin 43/GJA1 Antibody (BA1727) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. . Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• 

  Western blot analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (BA1727). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human placenta tissue lysates,
  Lane 2: rat brain tissue lysates,
  Lane 3: rat heart tissue lysates,
  Lane 4: mouse brain tissue lysates,
  Lane 5: mouse heart tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Connexin 43/GJA1 antigen affinity purified polyclonal antibody (BA1727) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Connexin 43/GJA1 at approximately 43 kDa. The expected band size for Connexin 43/GJA1 is at 43 kDa.

• 

  IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (BA1727).
  Connexin 43/GJA1 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Connexin 43/GJA1 Antibody (BA1727) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

• 

  IF analysis of Connexin 43/GJA1 using anti- Connexin 43/GJA1 antibody (BA1727).
  Connexin 43/GJA1 was detected in paraffin-embedded section of rat heart tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti- Connexin 43/GJA1 Antibody (BA1727) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. . Visualize using a fluorescence microscope and filter sets appropriate for the label used.