Western blot analysis of anti-Fibronectin(FN1) antibody (BA1771). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Fibronectin(FN1) antigen affinity purified polyclonal antibody (BA1771) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Fibronectin(FN1) at approximately 220-400 kDa. The expected band size for Fibronectin(FN1) is at 272 kDa.
IHC analysis of Fibronectin(FN1) using anti-Fibronectin(FN1) antibody (BA1771).
Fibronectin(FN1) was detected in a paraffin-embedded section of human adrenocortical adenoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Fibronectin(FN1) using anti-Fibronectin(FN1) antibody (BA1771).
Fibronectin(FN1) was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of CACO-2 cells using anti-Fibronectin(FN1) antibody (BA1771).
Overlay histogram showing CACO-2 cells stained with BA1771 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fibronectin(FN1) Antibody (BA1771, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of Hela cells using anti-Fibronectin(FN1) antibody (BA1771).
Overlay histogram showing Hela cells stained with BA1771 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fibronectin(FN1) Antibody (BA1771, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of anti-Fibronectin(FN1) antibody (BA1771). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Fibronectin(FN1) antigen affinity purified polyclonal antibody (BA1771) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Fibronectin(FN1) at approximately 220-400 kDa. The expected band size for Fibronectin(FN1) is at 272 kDa.
IHC analysis of Fibronectin(FN1) using anti-Fibronectin(FN1) antibody (BA1771).
Fibronectin(FN1) was detected in a paraffin-embedded section of human adrenocortical adenoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Fibronectin(FN1) using anti-Fibronectin(FN1) antibody (BA1771).
Fibronectin(FN1) was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of CACO-2 cells using anti-Fibronectin(FN1) antibody (BA1771).
Overlay histogram showing CACO-2 cells stained with BA1771 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fibronectin(FN1) Antibody (BA1771, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of Hela cells using anti-Fibronectin(FN1) antibody (BA1771).
Overlay histogram showing Hela cells stained with BA1771 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fibronectin(FN1) Antibody (BA1771, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Western blot analysis of anti-Fibronectin(FN1) antibody (BA1771). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Fibronectin(FN1) antigen affinity purified polyclonal antibody (BA1771) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Fibronectin(FN1) at approximately 220-400 kDa. The expected band size for Fibronectin(FN1) is at 272 kDa.
IHC analysis of Fibronectin(FN1) using anti-Fibronectin(FN1) antibody (BA1771).
Fibronectin(FN1) was detected in a paraffin-embedded section of human adrenocortical adenoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Fibronectin(FN1) using anti-Fibronectin(FN1) antibody (BA1771).
Fibronectin(FN1) was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of CACO-2 cells using anti-Fibronectin(FN1) antibody (BA1771).
Overlay histogram showing CACO-2 cells stained with BA1771 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fibronectin(FN1) Antibody (BA1771, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of Hela cells using anti-Fibronectin(FN1) antibody (BA1771).
Overlay histogram showing Hela cells stained with BA1771 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fibronectin(FN1) Antibody (BA1771, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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