Anti-Fibronectin/FN1 Antibody (Clone#EID-6)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM4460

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Fibronectin/FN1 antibody (BM4460). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Fibronectin/FN1 antigen affinity purified monoclonal antibody (BM4460) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Fibronectin/FN1 at approximately 263 kDa. The expected band size for Fibronectin/FN1 is at 263 kDa.
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Immunohistochemical analysis of paraffin-embedded human liver cancer, using Fibronectin Antibody.
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产品简介>

产品名称

Anti-Fibronectin/FN1 Antibody (Clone#EID-6)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CIG; Cold insoluble globulin; ED B; Fibronectin; fibronectin 1; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF

基因名称

FN1

克隆号

EID-6

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Fibronectin

计算分子量

263 kDa

实际分子量

273 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Fibronectin is a high-molecular weight glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. It is mapped to 2q35. This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.

Uniprot ID

P02751

文献引用格式

Fibronectin/FN1 Antibody (Clone#EID-6) (Boster Biological Technology, Wuhan, China. Catalog#BM4460)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

Fibronectin

实验方案>

实验步骤

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