Anti-NOX4 Antibody

筛选器： All WB IHC FCM

• 

  Western blot analysis of NOX4 using anti-NOX4 antibody (BA2813). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human SW620 whole cell lysates,
  Lane 3: human HK-2 whole cell lysates,
  Lane 4: human HL-60 whole cell lysates,
  Lane 5: human 293T whole cell lysates,
  Lane 6: human SW579 whole cell lysates,
  Lane 7: human SK-OV-3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NOX4 antigen affinity purified polyclonal antibody (BA2813) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NOX4 at approximately 65 kDa. The expected band size for NOX4 is at 67 kDa.

• 

  IHC analysis of NOX4 using anti-NOX4 antibody (BA2813).
  NOX4 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-NOX4 Antibody (BA2813) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

• 

  Flow Cytometry analysis of U2OS cells using anti-NOX4 antibody (BA2813).
  Overlay histogram showing U2OS cells stained with BA2813 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOX4 Antibody (BA2813) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of NOX4 using anti-NOX4 antibody (BA2813). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat kidney tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NOX4 antigen affinity purified polyclonal antibody (BA2813) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NOX4 at approximately 65 kDa. The expected band size for NOX4 is at 67 kDa.

• 

  Western blot analysis of NOX4 using anti-NOX4 antibody (BA2813). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human SW620 whole cell lysates,
  Lane 3: human HK-2 whole cell lysates,
  Lane 4: human HL-60 whole cell lysates,
  Lane 5: human 293T whole cell lysates,
  Lane 6: human SW579 whole cell lysates,
  Lane 7: human SK-OV-3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NOX4 antigen affinity purified polyclonal antibody (BA2813) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NOX4 at approximately 65 kDa. The expected band size for NOX4 is at 67 kDa.

• 

  IHC analysis of NOX4 using anti-NOX4 antibody (BA2813).
  NOX4 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-NOX4 Antibody (BA2813) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

• 

  Flow Cytometry analysis of U2OS cells using anti-NOX4 antibody (BA2813).
  Overlay histogram showing U2OS cells stained with BA2813 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOX4 Antibody (BA2813) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of NOX4 using anti-NOX4 antibody (BA2813). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat kidney tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NOX4 antigen affinity purified polyclonal antibody (BA2813) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NOX4 at approximately 65 kDa. The expected band size for NOX4 is at 67 kDa.