Anti-NOX4 Antibody (Clone#E-14)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP

BM4135

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-NOX4 antibody (BM4135). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U20S whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NOX4 antigen affinity purified monoclonal antibody (BM4135) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NOX4 at approximately 67 kDa. The expected band size for NOX4 is at 67 kDa.
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Immunohistochemical analysis of paraffin-embedded rat kidney, using NOX4 Antibody .
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis of HeLa cells, using NOX4 Antibody .
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产品简介>

产品名称

Anti-NOX4 Antibody (Clone#E-14)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Kidney oxidase 1; KOX; KOX 1; NADPH oxidase 4; NOX4; Renal NAD(P)H oxidase; RENOX

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP

基因名称

NOX4

克隆号

E-14

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human NOX4

计算分子量

67 kDa

实际分子量

65 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.

Uniprot ID

Q9NPH5

文献引用格式

NOX4 Antibody (Clone#E-14) (Boster Biological Technology, Wuhan, China. Catalog#BM4135)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

实验步骤

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