Anti-ZEB2 Antibody
送常用试剂

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA2872-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ZEB2 using anti-ZEB2 antibody (BA2872-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ZEB2 antigen affinity purified polyclonal antibody (BA2872-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ZEB2 at approximately 180 kDa. The expected band size for ZEB2 is at 136 kDa.
all(4)
IHC analysis of ZEB2 using anti-ZEB2 antibody (BA2872-2).
ZEB2 was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ZEB2 Antibody (BA2872-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of ZEB2 using anti-ZEB2 antibody (BA2872-2).
ZEB2 was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ZEB2 Antibody (BA2872-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of ZEB2 using anti-ZEB2 antibody (BA2872-2).
ZEB2 was detected in a paraffin-embedded section of human glioma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ZEB2 Antibody (BA2872-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)

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产品简介>

产品名称

Anti-ZEB2 Antibody

规格/价格

50μl|1280元
100μl|2180元
150μl|2800元

指标别称

KIAA0569; SIP 1; SIP1; Smad interacting protein 1; SMADIP1; ZEB2; ZFHX1B; ZFX1B

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

ZEB2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human ZEB2, identical to the related rat and mouse sequences.

计算分子量

136 kDa

实际分子量

180-200 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

ZEB2(Zinc finger E-box-binding homeobox2), also known as SIP1or ZINC FINGER HOMEOBOX 1B(ZFHX1B), is a protein that in humans is encoded by the ZEB2 gene. The ZEB2 gene is a member of the ZEB1/Drosophila Zfh1 family of 2-handed zinc finger/homeodomain proteins and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs, the transducers of TGF-beta signaling, and interacts with the nucleosome remodeling and histone deacetylation(NURD) complex. By radiation hybrid analysis, Nagase et al.(1998) mapped the ZEB2 gene to chromosome 2. Wakamatsu et al.(2001) mapped the ZEB2 gene to chromosome 2q22. Vandewalle et al.(2005) showed that expression of mouse Sip1 in human epithelial cells caused a morphologic change from an epithelial to a mesenchymal phenotype. Expression of SNAI1 in epithelial cells triggers an epithelial-mesenchyme transition. Beltran et al.(2008) showed that synthesis of ZEB2 was upregulated following SNAI1 expression in human cell lines.

Uniprot ID

O60315

文献引用格式

ZEB2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2872-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

zinc finger E-box binding homeobox 2

蛋白名全称

Zinc finger E-box-binding homeobox 2

实验方案>

实验步骤

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