Anti-ZEB2 Antibody (Clone#OTI15G8)
送常用试剂

Mouse monoclonal antibody

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筛选器： All WB IHC

M01169-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-ZEB2 antibody (M01169-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-ZEB2 antigen affinity purified monoclonal antibody (M01169-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ZEB2 at approximately 180 kDa. The expected band size for ZEB2 is at 136 kDa.
all(2)
IHC analysis of ZEB2 using anti-ZEB2 antibody (M01169-1).
ZEB2 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with mouse anti-ZEB2 Antibody (M01169-1) at a dilution of 1:200 and developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-ZEB2 Antibody (Clone#OTI15G8)

规格/价格

50μl|1280元
100μl|2180元
150μl|2800元

指标别称

KIAA0569; SIP 1; SIP1; Smad interacting protein 1; SMADIP1; ZEB2; ZFHX1B; ZFX1B

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

ZEB2

克隆号

OTI15G8

宿主

Mouse

抗体亚型

IgG1

免疫原

Human recombinant protein fragment corresponding to amino acids 1-350 of human ZEB2 (NP_055610) produced in E.coli.

计算分子量

136 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2010]

Uniprot ID

O60315

文献引用格式

ZEB2 Antibody (Clone#OTI15G8) (Boster Biological Technology, Wuhan, China. Catalog#M01169-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

zinc finger E-box binding homeobox 2

蛋白名全称

Zinc finger E-box-binding homeobox 2

实验方案>

实验步骤

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