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Anti-AMY1A Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

BA2973

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of AMY1A using anti-AMY1A antibody (BA2973). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human PANC-1 whole cell lysates,
    Lane 2: human PCP tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AMY1A antigen affinity purified polyclonal antibody (BA2973) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AMY1A at approximately 55-58 kDa. The expected band size for AMY1A is at 58 kDa.

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产品名称
Anti-AMY1A Antibody
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
amylase alpha 1A; AMY1; AMY1A
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC
基因名称
AMY1A
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the N-terminus of human Alpha Amylase 1, different from the related rat and mouse sequences by one amino acid.
计算分子量
58 kDa
实际分子量
58 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylase is present in human saliva, where it begins the chemical process of digestion. By in situ hybridization combined with high resolution cytogenetics, the amylase gene is mapped to 1p21.Amylase enzymes find use in bread making and to break down complex sugars such as starch(found in flour) into simple sugars. Yeast then feeds on these simple sugars and converts it into the waste products of alcohol and CO2.
Uniprot ID
P04745  
文献引用格式
AMY1A Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2973)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
amylase, alpha 1A(salivary)
蛋白名全称
Alpha-amylase 1
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-AMY1A Antibody

    筛选器: All WB IHC

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