Anti-CD90/THY1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA3391

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CD90/THY1 using anti-CD90/THY1 antibody (BA3391). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HT1080 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD90/THY1 antigen affinity purified polyclonal antibody (BA3391) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD90/THY1 at approximately 22-30 kDa. The expected band size for CD90/THY1 is at 18 kDa.
all(2)
IHC analysis of CD90/THY1 using anti-CD90/THY1 antibody (BA3391).
CD90/THY1 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD90/THY1 Antibody (BA3391) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-CD90/THY1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

T25; CD90; Thy-1; Thy1.1; Thy1.2; Thy-1.2; Thy-1 antigen; CDw90; Thy 1 antigen; Thy 1 cell surface antigen; Thy 1 membrane glycoprotein; THY1

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

THY1

宿主

Rabbit

抗体亚型

IgG

免疫原

Polypeptide

计算分子量

18 kDa

实际分子量

22-30 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CD90(Cluster of Differentiation 90) or Thy-1 is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol(GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. The CD90 gene is mapped to 11q23.3. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor.

Uniprot ID

P04216

文献引用格式

CD90/THY1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3391)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Thy-1 cell surface antigen

蛋白名全称

Thy-1 membrane glycoprotein

实验方案>

实验步骤

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