Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-200 |
Western blot analysis of anti-CD90/THY1 antibody (BM4210). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD90/THY1 antigen affinity purified monoclonal antibody (BM4210) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD90/THY1 at approximately 18 kDa. The expected band size for CD90/THY1 is at 18 kDa.
Immunohistochemical analysis of paraffin-embedded human tonsil, using CD90 / Thy1 Antibody.
Western blot analysis of anti-CD90/THY1 antibody (BM4210). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD90/THY1 antigen affinity purified monoclonal antibody (BM4210) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD90/THY1 at approximately 18 kDa. The expected band size for CD90/THY1 is at 18 kDa.
Immunohistochemical analysis of paraffin-embedded human tonsil, using CD90 / Thy1 Antibody.