| Western blot (WB): | 1:100-400 |
| Immunohistochemistry in paraffin section IHC-(P): | 1:50-200 |

Western blot analysis of IL2 using anti-IL2 antibody (BA3709). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL2 antigen affinity purified polyclonal antibody (BA3709) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL2 at approximately 18 kDa. The expected band size for IL2 is at 19 kDa.

Western blot analysis of IL2 using anti-IL2 antibody (BA3709). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL2 antigen affinity purified polyclonal antibody (BA3709) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL2 at approximately 18 kDa. The expected band size for IL2 is at 19 kDa.


