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Anti-IL2 Antibody

Rabbit polyclonal antibody

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筛选器: All WB

PB0059

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of IL2 using anti-IL2 antibody (PB0059). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: Rat Thymus tissue lysates,
    Lane 2: Rat Liver tissue lysates,
    Lane 3: NRK whole cell lysates,
    Lane 4: PC12 whole cell lysates,
    Lane 5: RH35 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL2 antigen affinity purified polyclonal antibody (PB0059) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL2 at approximately 18 kDa. The expected band size for IL2 is at 18 kDa.

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产品名称
Anti-IL2 Antibody
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
IL 2; IL2; IL-2; interleukin 2; T cell growth factor; TCGF
产品类型
Polyclonal
检验物种
rat
应用范围
WB
基因名称
IL2
宿主
Rabbit
抗体亚型
IgG
免疫原
E.coli-derived rat IL-2 recombinant protein (Position: A21-Q155). Rat IL-2 shares 66% and 80% amino acid (aa) sequence identity with human and mouse IL-2, respectively.
计算分子量
18 kDa
实际分子量
18 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. This gene for interleukin 2 (IL2) is assigned to chromosome 4. IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.
Uniprot ID
P17108  
文献引用格式
IL2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0059)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
interleukin 2
蛋白名全称
Interleukin-2
推荐稀释比
Western blot (WB):1:500-2000

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    Anti-IL2 Antibody

    筛选器: All WB

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