| Western blot (WB): | 1:500-2000 |

Western blot analysis of BMP4 using anti-BMP4 antibody (BA4669). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BMP4 antigen affinity purified polyclonal antibody (BA4669) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BMP4 at approximately 47 kDa. The expected band size for BMP4 is at 47 kDa.

Western blot analysis of BMP4 using anti-BMP4 antibody (BA4669). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BMP4 antigen affinity purified polyclonal antibody (BA4669) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BMP4 at approximately 47 kDa. The expected band size for BMP4 is at 47 kDa.
