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Anti-BMP4 Antibody (Clone#BBB-2)

Rabbit monoclonal antibody

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筛选器: All WB IHC ICC/IF IP FCM

BM4076

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti-BMP4 antibody (BM4076). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human COLO 320 whole cell lysates,
    Lane 3: human Caco-2 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BMP4 antigen affinity purified monoclonal antibody (BM4076) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BMP4 at approximately 47 kDa. The expected band size for BMP4 is at 47 kDa.

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产品名称
Anti-BMP4 Antibody (Clone#BBB-2)
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
BMP 2B; BMP 4; BMP2B; BMP2B1; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; MCOPS6; NeutraKine® BMP-4; ZYME
产品类型
Monoclonal
检验物种
human
应用范围
WB, IHC, ICC/IF, IP, FCM
基因名称
BMP4
克隆号
BBB-2
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human BMP4
计算分子量
47 kDa
实际分子量
47 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Bone morphogenetic protein 4 is a protein that in humans is encoded by BMP4 gene. It is found on chromosome 14q22-q23. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.
Uniprot ID
P12644  
文献引用格式
BMP4 Antibody (Clone#BBB-2) (Boster Biological Technology, Wuhan, China. Catalog#BM4076)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
Bone morphogenetic protein 4
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-200
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200
ImmunoPrecipitation (IP):1:20
Flow Cytometry (FCM):1:20

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    Anti-BMP4 Antibody (Clone#BBB-2)

    筛选器: All WB IHC ICC/IF IP FCM

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