Anti-CYP2E1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA4717

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CYP2E1 using anti-CYP2E1 antibody (BA4717). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CYP2E1 antigen affinity purified polyclonal antibody (BA4717) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CYP2E1 at approximately 57 kDa. The expected band size for CYP2E1 is at 57 kDa.
all(3)
IHC analysis of CYP2E1 using anti-CYP2E1 antibody (BA4717).
CYP2E1 was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CYP2E1 Antibody (BA4717) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)
IHC analysis of CYP2E1 using anti-CYP2E1 antibody (BA4717).
CYP2E1 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CYP2E1 Antibody (BA4717) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-CYP2E1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

4 nitrophenol 2 hydroxylase; CPE1; CYP2E; CYP2E1; CYP-2E1; CYP2E1-Specific; CYPIIE1; Cytochrome P450 2E1; Cytochrome P450 J; P450 J; P450C2E

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC

基因名称

CYP2E1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of mouse CYP2E1, identical to the related rat sequence.

计算分子量

57 kDa

实际分子量

57 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CYP2E1, also known as P450IIE1, is a member of the P450IIE subfamily which is ethanol-inducible. It has at least 1 gene which is mapped to 10q24.3-qter, and a second is likely in rat and in man. Both the rat and human proteins encoded by this gene contain 493 amino acids and calculated molecular masses of 56,634 and 56,916 daltons, respectively. In addition, genetic polymorphisms in the 5-prime flanking region of the human P450IIE1 gene affected its binding of transacting factor and changed its transcriptional regulation, which may lead to interindividual differences of microsomal drug oxidation activity. P450IIE1 is an important enzyme for the catalysis of the conversion of ethanol to acetaldehyde and to acetate in humans,and it is also involved in the metabolism of nitrosamines. Due to the possible correlation of P450IIE1 genes with malignancy, clinical studies of RFLP patterns of these genes in cancer may be useful.

Uniprot ID

Q05421

文献引用格式

CYP2E1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA4717)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cytochrome P450, family 2, subfamily E, polypeptide 1

蛋白名全称

Cytochrome P450 2E1

实验方案>

实验步骤

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