Anti-CYP2E1 Antibody (Clone#2C7G1)

Mouse monoclonal antibody

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筛选器： All WB

M00672-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CYP2E1 using anti-CYP2E1 antibody (M00672-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HCCP tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CYP2E1 antigen affinity purified monoclonal antibody (M00672-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CYP2E1 at approximately 57 kDa. The expected band size for CYP2E1 is at 57 kDa.
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产品名称

Anti-CYP2E1 Antibody (Clone#2C7G1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

4 nitrophenol 2 hydroxylase; CPE1; CYP2E; CYP2E1; CYP-2E1; CYP2E1-Specific; CYPIIE1; Cytochrome P450 2E1; Cytochrome P450 J; P450 J; P450C2E

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

基因名称

CYP2E1

克隆号

2C7G1

宿主

Mouse

抗体亚型

IgG2b

免疫原

E. coli-derived human Cytochrome P450 2E1/CYP2E1 recombinant protein (Position: H355-S493). Human CYP2E1 shares 86.3% amino acid (aa) sequence identity with both mouse and rat CYP2E1.

计算分子量

57 kDa

实际分子量

57 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Cytochrome P450 2E1 (abbreviated CYP2E1), a member of the cytochrome P450 mixed-function oxidase system, is involved in the metabolism of xenobiotics in the body. In humans, the CYP2E1 enzyme is encoded by the CYP2E1 gene. It is mapped to 10q26.3. While it is involved in the oxidative metabolism of a small range of substrates (mostly small polar molecules), there are many important drug interactions mediated by CYP2E1. Most drugs undergo deactivation by CYP2E1, either directly or by facilitated excretion from the body. Also, many substances are bioactivated by CYP2E1 to form their active compounds. In addition, CYP2E1 is an important enzyme for the conversion of ethanol to acetaldehyde and to acetate in humans. In the conversion sequence of acetyl-CoA to glucose, CYP2E1 transforms acetone via acetol into propylene glycol and methylglyoxal, the precursors of pyruvate, acetate and lactate.

Uniprot ID

P05181

文献引用格式

CYP2E1 Antibody (Clone#2C7G1) (Boster Biological Technology, Wuhan, China. Catalog#M00672-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cytochrome P450, family 2, subfamily E, polypeptide 1

蛋白名全称

Cytochrome P450 2E1

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