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Anti-Desmin/DES Antibody (Clone#DE-U-10)

Mouse monoclonal antibody

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筛选器: All WB IHC

BM0036

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti-Desmin antibody (BM0036). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat skeletal muscle tissue lysates,
    Lane 2: rat cardiac muscle tissue lysates,
    Lane 3: mouse skeletal muscle tissue lysates,
    Lane 4: mouse cardiac muscle tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Desmin antigen affinity purified monoclonal antibody (BM0036) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Desmin at approximately 54 kDa. The expected band size for Desmin is at 54 kDa.

    all(7)
  • IHC analysis of Desmin using anti-Desmin antibody (BM0036).
    Desmin was detected in a paraffin-embedded section of rat cardiac muscle tissue. The tissue section was developed using HRP Conjugated rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)
  • IHC analysis of Desmin using anti-Desmin antibody (BM0036).
    Desmin was detected in a paraffin-embedded section of human placenta tissue. The tissue section was developed using HRP Conjugated rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)
  • IHC analysis of Desmin using anti-Desmin antibody (BM0036).
    Desmin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was developed using HRP Conjugated rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)
  • IHC analysis of Desmin using anti-Desmin antibody (BM0036).
    Desmin was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was developed using HRP Conjugated rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)
  • IHC analysis of Desmin using anti-Desmin antibody (BM0036).
    Desmin was detected in a paraffin-embedded section of human placenta tissue. The tissue section was developed using HRP Conjugated rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)
  • IHC analysis of Desmin using anti-Desmin antibody (BM0036).
    Desmin was detected in a paraffin-embedded section of human invasive urothelial carcinoma tissue. The tissue section was developed using HRP Conjugated rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)

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产品名称
Anti-Desmin/DES Antibody (Clone#DE-U-10)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CMD1I; CSM1; CSM2; Cytoskeleton Marker; DES; desmin
产品类型
Monoclonal
检验物种
human, mouse, rat, rabbit
应用范围
WB, IHC
基因名称
DES
克隆号
DE-U-10
宿主
Mouse
抗体亚型
IgG1
免疫原
Desmin from pig stomach.
计算分子量
54 kDa
实际分子量
54 kDa
成分
200ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式
Ascites
浓度
200 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Desmin belongs to the type III family of intermediate filaments, a class of cytoskeletal elements. DES gene encodes desmin, a muscle-specific cytoskeletal protein found in smooth, cardiac, and heart muscles. Tidball(1992) found that desmin was codistributed with actin thin filaments within the cellular processes of myotendinous junctions in frog skeletal muscle. DES gene contains 9 exons and spans about 8.4 kb. By in situ hybridization, Viegas-Pequignot et al.(1989) localized the gene to 2q35. Desmin mutation responsible for idiopathic dilated cardiomyopathy.
Uniprot ID
P17661  
文献引用格式
Desmin/DES Antibody (Clone#DE-U-10) (Boster Biological Technology, Wuhan, China. Catalog#BM0036)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
基因名全称
desmin
蛋白名全称
Desmin
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-Desmin/DES Antibody (Clone#DE-U-10)

    筛选器: All WB IHC

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