Western blot analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse skeletal muscle tissue lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Desmin/DES antigen affinity purified monoclonal antibody (M01948-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Desmin/DES at approximately 54 kDa. The expected band size for Desmin/DES is at 54 kDa.
IHC analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3).
Desmin/DES was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Desmin/DES Antibody (M01948-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3).
Desmin/DES was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Desmin/DES Antibody (M01948-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3).
Desmin/DES was detected in a paraffin-embedded section of human skeletal muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Desmin/DES Antibody (M01948-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3).
Desmin/DES was detected in a paraffin-embedded section of rat skeletal muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Desmin/DES Antibody (M01948-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3).
Desmin/DES was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Desmin/DES Antibody (M01948-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of THP-1 cells using anti-Desmin/DES antibody (M01948-3).
Overlay histogram showing THP-1 cells stained with M01948-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Desmin/DES Antibody (M01948-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse skeletal muscle tissue lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Desmin/DES antigen affinity purified monoclonal antibody (M01948-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Desmin/DES at approximately 54 kDa. The expected band size for Desmin/DES is at 54 kDa.
IHC analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3).
Desmin/DES was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Desmin/DES Antibody (M01948-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3).
Desmin/DES was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Desmin/DES Antibody (M01948-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3).
Desmin/DES was detected in a paraffin-embedded section of human skeletal muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Desmin/DES Antibody (M01948-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3).
Desmin/DES was detected in a paraffin-embedded section of rat skeletal muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Desmin/DES Antibody (M01948-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3).
Desmin/DES was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Desmin/DES Antibody (M01948-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of THP-1 cells using anti-Desmin/DES antibody (M01948-3).
Overlay histogram showing THP-1 cells stained with M01948-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Desmin/DES Antibody (M01948-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse skeletal muscle tissue lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Desmin/DES antigen affinity purified monoclonal antibody (M01948-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Desmin/DES at approximately 54 kDa. The expected band size for Desmin/DES is at 54 kDa.
IHC analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3).
Desmin/DES was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Desmin/DES Antibody (M01948-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3).
Desmin/DES was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Desmin/DES Antibody (M01948-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3).
Desmin/DES was detected in a paraffin-embedded section of human skeletal muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Desmin/DES Antibody (M01948-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3).
Desmin/DES was detected in a paraffin-embedded section of rat skeletal muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Desmin/DES Antibody (M01948-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Desmin/DES using anti-Desmin/DES antibody (M01948-3).
Desmin/DES was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Desmin/DES Antibody (M01948-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of THP-1 cells using anti-Desmin/DES antibody (M01948-3).
Overlay histogram showing THP-1 cells stained with M01948-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Desmin/DES Antibody (M01948-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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