Anti-PI3 Kinase p110 Alpha/PIK3CA Antibody (Clone#BEB-16)

Rabbit monoclonal antibody

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筛选器： All WB ICC/IF IP

BM4107

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of PI 3 kinase p110 alpha expression in Jurkat cell lysate.Western blot analysis of PI 3 kinase p110 alpha expression in Jurkat cell lysate.
all(6)
All lanes use the Antibody for 1 hour at room temperature.
all(6)
All lanes use the Antibody for 1 hour at room temperature.
all(6)
All lanes use the Antibody for 1 hour at room temperature.
all(6)
all(6)
all(6)

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产品简介>

产品名称

Anti-PI3 Kinase p110 Alpha/PIK3CA Antibody (Clone#BEB-16)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

p110 alpha; PI3 kinase subunit alpha; PI3K; PI3K alpha; PI3K p110(alpha); PIK3CA; PtdIns 3 kinase subunit alpha

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF, IP

基因名称

PIK3CA

克隆号

BEB-16

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human PI 3 Kinase catalytic subunit alpha Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

计算分子量

124 kDa

实际分子量

110 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Phosphatidylinositol-4,5-bisphosphate 3-kinase, also called PIK3CA, is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. PIK3CA gene is mapped to 3q26.32. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate phosphatidylinositols (PtdIns), PtdIns4P andPtdIns(4,5)P2. Recent evidence has shown that the PIK3CA gene is mutated in a range of human cancers. It has been found to be oncogenic and has been implicated in cervical cancers. PIK3CA mutations in breast cancer may be a predictive marker to guide the selection of patients who would benefit from mTOR inhibitor therapy. In addition to that, the presence of PIK3CA mutation may predict response to aspirin therapy for colorectal cancer, indicating power and promise of "Molecular Pathological Epidemiology (MPE)" approach as well as a complex interaction within the tumor microenvironment in this phenomenon.

Uniprot ID

P42336

文献引用格式

PI3 Kinase p110 Alpha/PIK3CA Antibody (Clone#BEB-16) (Boster Biological Technology, Wuhan, China. Catalog#BM4107)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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