Anti-PI3 Kinase p110 Alpha/PIK3CA Antibody

筛选器： All WB FCM

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  Western blot analysis of PI3 Kinase p110 Alpha/PIK3CA using anti-PI3 Kinase p110 Alpha/PIK3CA antibody (PB0351). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Jurkat whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates,
  Lane 3: human K562 whole cell lysates,
  Lane 4: human Raji whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PI3 Kinase p110 Alpha/PIK3CA antigen affinity purified polyclonal antibody (PB0351) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PI3 Kinase p110 Alpha/PIK3CA at approximately 124 kDa. The expected band size for PI3 Kinase p110 Alpha/PIK3CA is at 124 kDa.

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  Flow Cytometry analysis of Raji cells using anti-PI3 Kinase p110 Alpha/PIK3CA antibody (PB0351).
  Overlay histogram showing Raji cells stained with PB0351 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PI3 Kinase p110 Alpha/PIK3CA Antibody (PB0351) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of PI3 Kinase p110 Alpha/PIK3CA using anti-PI3 Kinase p110 Alpha/PIK3CA antibody (PB0351). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Jurkat whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates,
  Lane 3: human K562 whole cell lysates,
  Lane 4: human Raji whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PI3 Kinase p110 Alpha/PIK3CA antigen affinity purified polyclonal antibody (PB0351) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PI3 Kinase p110 Alpha/PIK3CA at approximately 124 kDa. The expected band size for PI3 Kinase p110 Alpha/PIK3CA is at 124 kDa.

• 

  Flow Cytometry analysis of Raji cells using anti-PI3 Kinase p110 Alpha/PIK3CA antibody (PB0351).
  Overlay histogram showing Raji cells stained with PB0351 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PI3 Kinase p110 Alpha/PIK3CA Antibody (PB0351) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.