Anti-c-Kit/CD117/KIT Antibody (Clone#CGF-11)
送常用试剂

Rabbit monoclonal antibody

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筛选器： All WB IHC

BM4234

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-c-Kit/CD117/KIT antibody (BM4234). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-c-Kit/CD117/KIT antigen affinity purified monoclonal antibody (BM4234) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for c-Kit/CD117/KIT at approximately 145 kDa. The expected band size for c-Kit/CD117/KIT is at 110 kDa.
all(2)
Immunohistochemical analysis of paraffin-embedded human breast cancer, using c-Kit Antibody .
all(2)

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产品简介>

产品名称

Anti-c-Kit/CD117/KIT Antibody (Clone#CGF-11)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

C Kit; CD117; c-Kit; c-Kit/CD117; KIT; PBT; Proto oncogene c Kit; SCFR; Tyrosine protein kinase Kit

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC

基因名称

KIT

克隆号

CGF-11

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human c-Kit

计算分子量

110 kDa

实际分子量

145 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

SCFR(Mast/stem cell growth factor receptor), also known as KIT ONCOGENE or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

Uniprot ID

P10721

文献引用格式

c-Kit/CD117/KIT Antibody (Clone#CGF-11) (Boster Biological Technology, Wuhan, China. Catalog#BM4234)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Mast/stem cell growth factor receptor Kit

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实验步骤

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