Anti-c-Kit/CD117/KIT Antibody
送常用试剂

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM

PB9258

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of c-Kit/CD117/KIT using anti-c-Kit/CD117/KIT antibody (PB9258).
Lane 1: recombinant Human C-Kit Protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-c-Kit/CD117/KIT antigen affinity purified polyclonal antibody (PB9258) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for c-Kit/CD117/KIT at approximately 145 kDa.
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Western blot analysis of c-Kit/CD117/KIT using anti-c-Kit/CD117/KIT antibody (PB9258). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HEPG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-c-Kit/CD117/KIT antigen affinity purified polyclonal antibody (PB9258) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for c-Kit/CD117/KIT at approximately 145 kDa. The expected band size for c-Kit/CD117/KIT is at 110 kDa.
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IHC analysis of c-Kit/CD117/KIT using anti-c-Kit/CD117/KIT antibody (PB9258).
c-Kit/CD117/KIT was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-c-Kit/CD117/KIT Antibody (PB9258) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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Flow Cytometry analysis of K562 cells using anti-c-Kit/CD117/KIT antibody (PB9258).
Overlay histogram showing K562 cells stained with PB9258 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-c-Kit/CD117/KIT Antibody (PB9258) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介>

产品名称

Anti-c-Kit/CD117/KIT Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

C Kit; CD117; c-Kit; c-Kit/CD117; KIT; PBT; Proto oncogene c Kit; SCFR; Tyrosine protein kinase Kit

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, FCM

基因名称

KIT

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human c-Kit recombinant protein (Position: Q26-S285). Human c-Kit shares 66% amino acid (aa) sequence identity with mouse c-Kit.

计算分子量

110 kDa

实际分子量

145 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

SCFR(Mast/stem cell growth factor receptor), also known as KIT ONCOGENE or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

Uniprot ID

P10721

RRID

AB_3082039

文献引用格式

c-Kit/CD117/KIT Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9258)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

蛋白名全称

Mast/stem cell growth factor receptor Kit

实验方案>

实验步骤

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