Anti-TOM20/TOMM20 Antibody (Clone#EOC-20)

Rabbit monoclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF FCM

BM4366

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of anti-TOM20/TOMM20 antibody (BM4366). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat stomach tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse stomach tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TOM20/TOMM20 antigen affinity purified monoclonal antibody (BM4366) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TOM20/TOMM20 at approximately 16 kDa. The expected band size for TOM20/TOMM20 is at 16 kDa.
all(5)
IHC analysis of TOM22/TOMM22 using anti-TOM22/TOMM22 antibody (BM4366) .
TOM22/TOMM22 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-TOM22/TOMM22 Antibody (BM4366) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of TOM22/TOMM22 using anti-TOM22/TOMM22 antibody (BM4366) .
TOM22/TOMM22 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-TOM22/TOMM22 Antibody (BM4366) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
Immunofluorescent analysis of Hela cells, using TOMM20 Antibody.
all(5)
all(5)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-TOM20/TOMM20 Antibody (Clone#EOC-20)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Cell and organelle markers; KIAA0016; MAS20; Mitochondria Marker; Mitochondrial Marker; Mitochondrion Marker; MOM19; tom 20; TOM20; TOMM20

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

TOMM20

克隆号

EOC-20

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human TOMM20

计算分子量

16 kDa

实际分子量

16 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.

Uniprot ID

Q15388

文献引用格式

TOM20/TOMM20 Antibody (Clone#EOC-20) (Boster Biological Technology, Wuhan, China. Catalog#BM4366)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Mitochondrial import receptor subunit TOM20 homolog

实验方案>

实验步骤

引用文献(0)>

清除