Anti-FOXO3A/FOXO3 (Phospho-S253) Antibody (Clone#ECG-6)

Rabbit Monoclonal antibody

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筛选器： All WB IHC

BM4401

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of FOXO3A/FOXO3 (Phospho-S253) using anti-FOXO3A/FOXO3 (Phospho-S253) antibody (BM4401). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human T-47D whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FOXO3A/FOXO3 (Phospho-S253) antigen affinity purified monoclonal antibody (BM4401) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FOXO3A/FOXO3 (Phospho-S253) at approximately 80-100 kDa. The expected band size for FOXO3A/FOXO3 (Phospho-S253) is at 71 kDa.
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产品简介>

产品名称

Anti-FOXO3A/FOXO3 (Phospho-S253) Antibody (Clone#ECG-6)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

AF6q21; AF6q21 protein; FKHRL1; FKHRL1P2; forkhead box O3; Forkhead box protein O3; FOXO2; FOXO3; FOXO3A

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

FOXO3

克隆号

ECG-6

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human FoxO3a

计算分子量

71 kDa

实际分子量

80-100 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

Uniprot ID

O43524

文献引用格式

FOXO3A/FOXO3 (Phospho-S253) Antibody (Clone#ECG-6) (Boster Biological Technology, Wuhan, China. Catalog#BM4401)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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