Anti-PPP2CA/B Antibody (Clone#ECI-16)

Rabbit monoclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF IP

BM4403

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of anti- PPP2CA antibody (BM4403). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: 293T whole cell, Lane 2: Hela whole cell, Lane 3: rat liver tissue, Lane 4: C6 whole cell, Lane 5: mouse liver tissue, Lane 6: NIH/3T3 whole cell. Use mouse anti- PPP2CA 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for PPP2CA at approximately 35KD. The expected band size for PPP2CA is at 35KD.
all(4)
all(4)
all(4)
all(4)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-PPP2CA/B Antibody (Clone#ECI-16)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

PP2A alpha; PP2Ac; PP2CA; PPP2CA; Replication protein C; RP C; PP2A beta; PP2CB; PPP2CB

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP

基因名称

PPP2CA/PPP2CB

克隆号

ECI-16

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human alpha + beta PP2A

计算分子量

35 kDa

实际分子量

36 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A groupof proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit, and a catalyticsubunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family. The PP2A family comprises subfamily members PP2Aα and PP2Aβ. The PP2A catalytic subunit associates with a variety of regulatory subunits. Regulatory subunits include PP2A-Aα and -Aβ, PP2A-Bα and -Bβ, PP2A-Cα and -Cβ, PP2A-B56α and -B56β. Reversible carboxymethylation of the C-terminal leucine residue (Leu309) of the PP2A catalytic subunit (PP2A-Cα/β) in vitro has been shown to regulate catalytic activity. It has been suggested that this posttranslational modification may play a role in assembly of the holoenzyme.

Uniprot ID

P62714/P67775

文献引用格式

PPP2CA/B Antibody (Clone#ECI-16) (Boster Biological Technology, Wuhan, China. Catalog#BM4403)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform

实验方案>

实验步骤

引用文献(0)>

清除