产品名称
Anti-pro Caspase 10/CASP10 Antibody (Clone#GAF-3)
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
ALPS2; Apoptotic protease Mch 4; CASP 10; CASP10; Caspase 10; Caspase 10/p23/17/p12; Caspase10; FLICE2; ICE like apoptotic protease 4; MCH4
应用范围
WB, ICC/IF, IHC, IP, FCM
免疫原
A synthesized peptide derived from human pro Caspase 10
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
保存条件
12 months from date of receipt，-20℃ as supplied.
背景资料
Caspase-10 is an enzyme that, in humans, is encoded by the CASP10 gene. The Caspase 10 gene contains 11 exons and spans about 48 kb. This gene is mapped to 2q33.1. It is transcribed in the centromere-to-telomere direction.This gene encodes a protein that is a member of the cysteine-aspartic acid protease(caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene.
文献引用格式
pro Caspase 10/CASP10 Antibody (Clone#GAF-3) (Boster Biological Technology, Wuhan, China. Catalog#BM4584)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐稀释比
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
| ImmunoPrecipitation (IP): | 1:10 |
| Flow Cytometry (FCM): | 1:50 |
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