Anti-Caspase 10/CASP10 (N-terminal) Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA3192

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Caspase 10/CASP10 using anti-Caspase 10/CASP10 antibody (BA3192). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: COLO320 whole cell lysates,
Lane 2: HELA whole cell lysates,
Lane 3: SW620 whole cell lysates,
Lane 4: RAJI whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspase 10/CASP10 antigen affinity purified polyclonal antibody (BA3192) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspase 10/CASP10 at approximately 59 kDa. The expected band size for Caspase 10/CASP10 is at 59 kDa.
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IHC analysis of Caspase 10/CASP10 using anti-Caspase 10/CASP10 antibody (BA3192).
Caspase 10/CASP10 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-Caspase 10/CASP10 Antibody (BA3192) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品名称

Anti-Caspase 10/CASP10 (N-terminal) Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

ALPS2; Apoptotic protease Mch 4; CASP 10; CASP10; Caspase 10; Caspase 10/p23/17/p12; Caspase10; FLICE2; ICE like apoptotic protease 4; MCH4

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

CASP10

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human CASP10.

计算分子量

59 kDa

实际分子量

59 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Caspase-10 is an enzyme that, in humans, is encoded by the CASP10 gene. The Caspase 10 gene contains 11 exons and spans about 48 kb. This gene is mapped to 2q33.1. It is transcribed in the centromere-to-telomere direction.This gene encodes a protein that is a member of the cysteine-aspartic acid protease(caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Uniprot ID

Q92851

文献引用格式

Caspase 10/CASP10 (N-terminal) Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3192)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

caspase 10, apoptosis-related cysteine peptidase

蛋白名全称

Caspase-10(CASP-10)

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