| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
| ImmunoPrecipitation (IP): | 1:40 |
| Flow Cytometry (FCM): | 1:100 |

Western blot analysis of anti-S100A4 antibody (BM4636). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-S100A4 antigen affinity purified monoclonal antibody (BM4636) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for S100A4 at approximately 12 kDa. The expected band size for S100A4 is at 12 kDa.

Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody.

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody.

Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma, using the Antibody.

Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using S100A4 Antibody.

Immunofluorescent analysis of HeLa cells, using S100A4 Antibody.

Western blot analysis of anti-S100A4 antibody (BM4636). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-S100A4 antigen affinity purified monoclonal antibody (BM4636) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for S100A4 at approximately 12 kDa. The expected band size for S100A4 is at 12 kDa.

Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody.

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody.

Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma, using the Antibody.

Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using S100A4 Antibody.

Immunofluorescent analysis of HeLa cells, using S100A4 Antibody.










