Anti-SMAD2 (Phospho-S250) Antibody (Clone#HBC-19)

Rabbit monoclonal antibody

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筛选器： All WB FCM

BM4693

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-SMAD2 (Phospho-S250) antibody (BM4693). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human HT-1080 whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat skeletal muscle tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SMAD2 (Phospho-S250) antigen affinity purified monoclonal antibody (BM4693) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SMAD2 (Phospho-S250) at approximately 58 kDa. The expected band size for SMAD2 (Phospho-S250) is at 52 kDa.
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产品名称

Anti-SMAD2 (Phospho-S250) Antibody (Clone#HBC-19)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

hMAD 2; hSMAD2; JV18; JV18 1; MAD homolog 2; Mad related protein 2; MADH2; MADR2; Mothers against DPP homolog 2; SMAD 2; SMAD family member 2; SMAD2

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, FCM

基因名称

SMAD2

克隆号

HBC-19

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Smad2

计算分子量

52 kDa

实际分子量

58 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Smad2(Mothers against decapentaplegic homolog 2), also known as MADR2, MADH2, SMAD family member 2 or SMAD2, is a protein that in humans is encoded by the SMAD2 gene. MAD homolog 2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic'(Mad) and the C. elegans gene Sma. Eppert et al.(1996) mapped the MADR2 gene close to DPC4 at 18q21, a region which is frequently deleted in colorectal cancers. Riggins et al.(1996) mapped the human MADH2 gene to 18q21. Nakao et al.(1997) refined the localization of the SMAD2 gene to 18q21.1, approximately 3 Mb proximal to DPC4, by fluorescence in situ hybridization. SMAD2 mediates the signal of the transforming growth factor(TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation(SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors.

Uniprot ID

Q15796

文献引用格式

SMAD2 (Phospho-S250) Antibody (Clone#HBC-19) (Boster Biological Technology, Wuhan, China. Catalog#BM4693)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Mothers against decapentaplegic homolog 2

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