Anti-SMAD2 Antibody (Clone#OTI2C10)

Mouse monoclonal antibody

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筛选器： All WB FCM ICC/IF

M00090-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-SMAD2 antibody (M00090-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: mouse skeletal muscle lysates,
Lane 6: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-SMAD2 antigen affinity purified monoclonal antibody (M00090-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SMAD2 at approximately 52 kDa. The expected band size for SMAD2 is at 52 kDa.
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Flow cytometric analysis of living 293T cells transfected with SMAD2 overexpression plasmid , Red)/empty vector (Blue) using anti-SMAD2 antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).
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Flow cytometric Analysis of permeabilized HUVEC cells, using anti-SMAD2 antibody, (Red), compared to an IgG isotype control, (green) (1:100).
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Flow cytometric Analysis of Jurkat cells, using anti-SMAD2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
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Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY SMAD2 using anti-SMAD2 antibody. 293T cells transfected with empty vector served as a negative control (right) (1:100).
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产品简介>

产品名称

Anti-SMAD2 Antibody (Clone#OTI2C10)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

hMAD 2; hSMAD2; JV18; JV18 1; MAD homolog 2; Mad related protein 2; MADH2; MADR2; Mothers against DPP homolog 2; SMAD 2; SMAD family member 2; SMAD2

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, FCM, ICC/IF

基因名称

SMAD2

克隆号

OTI2C10

宿主

Mouse

抗体亚型

IgG2b

免疫原

Full length human recombinant protein of human SMAD2 (NP_005892) produced in HEK293T cell.

计算分子量

52 kDa

实际分子量

52 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Smad2(Mothers against decapentaplegic homolog 2), also known as MADR2, MADH2, SMAD family member 2 or SMAD2, is a protein that in humans is encoded by the SMAD2 gene. MAD homolog 2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. Eppert et al. mapped the MADR2 gene close to DPC4 at 18q21, a region which is frequently deleted in colorectal cancers. Riggins et al. mapped the human MADH2 gene to 18q21. Nakao et al. refined the localization of the SMAD2 gene to 18q21.1, approximately 3 Mb proximal to DPC4, by fluorescence in situ hybridization. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors.

Uniprot ID

Q15796

文献引用格式

SMAD2 Antibody (Clone#OTI2C10) (Boster Biological Technology, Wuhan, China. Catalog#M00090-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

SMAD family member 2

蛋白名全称

Mothers against decapentaplegic homolog 2

实验方案>

实验步骤

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