Anti-STIM1 Antibody (Clone#HEC-19)

Rabbit monoclonal antibody

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筛选器： All WB IHC IP

BM4723

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-STIM1 antibody (BM4723). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human HUH-7 whole cell lysates,
Lane 5: rat RH-35 whole cell lysates,
Lane 6: mouse Hepa1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STIM1 antigen affinity purified monoclonal antibody (BM4723) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for STIM1 at approximately 85 kDa. The expected band size for STIM1 is at 77 kDa.
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产品简介>

产品名称

Anti-STIM1 Antibody (Clone#HEC-19)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

D11S4896E; GOK; STIM1; stromal interaction molecule 1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IP

基因名称

STIM1

克隆号

HEC-19

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Stromal interaction molecule 1

计算分子量

77 kDa

实际分子量

85-97 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Stromal interaction molecule 1 is a protein that in humans is encoded by the STIM1 gene. STIM1 has a single transmembranedomain, and is localized to the endoplasmic reticulum, and to a lesser extent to the plasma membrane. This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants.

Uniprot ID

Q13586

文献引用格式

STIM1 Antibody (Clone#HEC-19) (Boster Biological Technology, Wuhan, China. Catalog#BM4723)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Stromal interaction molecule 1

实验方案>

实验步骤

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