Anti-STIM1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

PB9406

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of STIM1 using anti-STIM1 antibody (PB9406). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: jurkat whole cell lysates,
Lane 2: K562 whole cell lysates,
Lane 3: HEPG2 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: RH35 whole cell lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STIM1 antigen affinity purified polyclonal antibody (PB9406) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for STIM1 at approximately 85-97 kDa. The expected band size for STIM1 is at 77 kDa.
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IHC analysis of STIM1 using anti-STIM1 antibody (PB9406).
STIM1 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-STIM1 Antibody (PB9406) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of STIM1 using anti-STIM1 antibody (PB9406).
STIM1 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-STIM1 Antibody (PB9406) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of STIM1 using anti-STIM1 antibody (PB9406).
STIM1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-STIM1 Antibody (PB9406) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IF analysis of STIM1 using anti-STIM1 antibody (PB9406).
STIM1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-STIM1 Antibody (PB9406) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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产品简介>

产品名称

Anti-STIM1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

D11S4896E; GOK; STIM1; stromal interaction molecule 1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

STIM1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human STIM1, identical to the related mouse and rat sequences.

计算分子量

77 kDa

实际分子量

85-97 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Stromal interaction molecule 1 is a protein that in humans is encoded by the STIM1 gene. STIM1 has a single transmembranedomain, and is localized to the endoplasmic reticulum, and to a lesser extent to the plasma membrane. This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants.

Uniprot ID

Q13586

文献引用格式

STIM1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9406)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

stromal interaction molecule 1

蛋白名全称

Stromal interaction molecule 1

实验方案>

实验步骤

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