| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |

Western blot analysis of anti- KLF4 antibody (BM4916). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: PC-3 whole cell, Lane 2: A431 whole cell, Lane 3: Hela whole cell, Lane 4: C6 whole cell, Lane 5: NIH/3T3 whole cell. Use mouse anti- KLF4 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for KLF4 at approximately 55KD. The expected band size for KLF4 is at 54KD.

Western blot analysis of anti- KLF4 antibody (BM4916). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: PC-3 whole cell, Lane 2: A431 whole cell, Lane 3: Hela whole cell, Lane 4: C6 whole cell, Lane 5: NIH/3T3 whole cell. Use mouse anti- KLF4 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for KLF4 at approximately 55KD. The expected band size for KLF4 is at 54KD.




