Anti-AHR Antibody (Clone#AOHI-1)

Rabbit monoclonal antibody

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筛选器： All WB ICC/IF

BM4965

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-AHR antibody (BM4965). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AHR antigen affinity purified monoclonal antibody (BM4965) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AHR at approximately 96, 105 kDa. The expected band size for AHR is at 96 kDa.
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产品简介>

产品名称

Anti-AHR Antibody (Clone#AOHI-1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Ah receptor; AHR; aryl hydrocarbon receptor; bHLHe76

产品类型

Monoclonal

检验物种

human

应用范围

WB, ICC/IF

基因名称

AHR

克隆号

AOHI-1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human AhR

计算分子量

96 kDa

实际分子量

100 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

AHR (aryl hydrocarbon receptor), also called bHLHe76, is a member of the family of basic helix-loop-helix transcription factors. AhR is a cytosolic transcription factor that is normally inactive, bound to several co-chaperones. The AHR gene is mapped on 7p21.1. Estrogenic actions of AHR agonists were detected in wildtype ovariectomized mouse uteri, but were absent in Ahr -/- or Er-alpha -/- ovariectomized mice. Complex assembly and ubiquitin ligase activity of CUL4B(AHR) in vitro and in vivo are dependent on the AHR ligand. In the CUL4B(AHR) complex, ligand-activated AHR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Cd4-positive cells from mice lacking Ahr developed Th17 responses but failed to produce Il22 and did not show enhanced Th17 development. Activation of Ahr during induction of EAE accelerated disease onset and increased pathology in wildtype mice, but not in Ahr -/- mice. The TDO-AHR pathway is active in human brain tumors and is associated with malignant progression and poor survival. Ahr activity within ROR-gamma-t-positive ILC could be induced by dietary ligands such as those contained in vegetables of the family Brassicaceae.

Uniprot ID

P35869

文献引用格式

AHR Antibody (Clone#AOHI-1) (Boster Biological Technology, Wuhan, China. Catalog#BM4965)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Aryl hydrocarbon receptor

实验方案>

实验步骤

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