Anti-P-Selectin/SELP Antibody (Clone#AAEC-19)

Rabbit monoclonal antibody

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筛选器： All WB IHC

BM5032

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-P-Selectin/SELP antibody (BM5032). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human Jurkat whole cell lysates,
Lane 7: human SIHA whole cell lysates,
Lane 8: human RT4 whole cell lysates,
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P-Selectin/SELP antigen affinity purified monoclonal antibody (BM5032) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P-Selectin/SELP at approximately 91 kDa. The expected band size for P-Selectin/SELP is at 91 kDa.
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产品简介>

产品名称

Anti-P-Selectin/SELP Antibody (Clone#AAEC-19)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD62; CD62P; GMP 140; GMP140; GMRP; Granule membrane protein 140; GRMP; LECAM3; P selectin; PADGEM; PSEL; P-selectin; SELP

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC

基因名称

SELP

克隆号

AAEC-19

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human CD62P

计算分子量

91 kDa

实际分子量

140 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CD62P is also known as SELP or P-selectin. This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented.

Uniprot ID

P16109

文献引用格式

P-Selectin/SELP Antibody (Clone#AAEC-19) (Boster Biological Technology, Wuhan, China. Catalog#BM5032)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

P-selectin

实验方案>

实验步骤

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