Anti-P-Selectin/SELP Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA0617

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of P-Selectin/SELP using anti-P-Selectin/SELP antibody (BA0617). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PANC whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P-Selectin/SELP antigen affinity purified polyclonal antibody (BA0617) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P-Selectin/SELP at approximately 140 kDa. The expected band size for P-Selectin/SELP is at 83 kDa.
all(2)
IHC analysis of P-Selectin/SELP using anti-P-Selectin/SELP antibody (BA0617).
P-Selectin/SELP was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-P-Selectin/SELP Antibody (BA0617) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-P-Selectin/SELP Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD62; CD62P; GMP 140; GMP140; GMRP; Granule membrane protein 140; GRMP; LECAM3; P selectin; PADGEM; PSEL; P-selectin; SELP

产品类型

Polyclonal

检验物种

human, mouse, rat, rabbit

应用范围

WB, IHC

基因名称

Selp

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived mouse CD62P recombinant protein (Position: W42-A267).

计算分子量

83 kDa

实际分子量

140 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CD62P is also known as SELP or P-selectin. This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented.

Uniprot ID

Q01102

文献引用格式

P-Selectin/SELP Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0617)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

selectin, platelet

蛋白名全称

P-selectin

实验方案>

实验步骤

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