| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
| Flow Cytometry (FCM): | 1:20 |

Western blot analysis of anti-NR1D1 antibody (BM5531). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat RH-35 whole cell lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NR1D1 antigen affinity purified monoclonal antibody (BM5531) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NR1D1 at approximately 55 kDa. The expected band size for NR1D1 is at 67 kDa.

Western blot analysis of anti-NR1D1 antibody (BM5531). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat RH-35 whole cell lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NR1D1 antigen affinity purified monoclonal antibody (BM5531) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NR1D1 at approximately 55 kDa. The expected band size for NR1D1 is at 67 kDa.






