Anti-OGT Antibody (Clone#AEDG-15)

Rabbit monoclonal antibody

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筛选器： All WB ICC/IF IHC FCM

BM5564

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-OGT antibody (BM5564). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OGT antigen affinity purified monoclonal antibody (BM5564) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for OGT at approximately 110 kDa. The expected band size for OGT is at 117 kDa.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis using the Antibody.
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产品简介>

产品名称

Anti-OGT Antibody (Clone#AEDG-15)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

HRNT1; OGT

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF, IHC, FCM

基因名称

OGT

克隆号

AEDG-15

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human OGT / O-Linked N-Acetylglucosamine Transferase Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.

计算分子量

117 kDa

实际分子量

110 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) is an enzyme that in humans is encoded by the OGT gene. This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Uniprot ID

O15294

文献引用格式

OGT Antibody (Clone#AEDG-15) (Boster Biological Technology, Wuhan, China. Catalog#BM5564)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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