产品名称
Anti-CKM/CKB Antibody (Clone#AFOC-3)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
B CK; CKB; CKB/CKM; CKBB; Creatine kinase B chain; Creatine kinase B type; creatine kinase; brain
应用范围
WB, IHC, ICC/IF, IP, FCM
免疫原
A synthesized peptide derived from human Creatine kinase B type Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) . Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Brain-type creatine kinase also known as CK-BB is a creatine kinase that in humans is encoded by the CKB gene. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.
文献引用格式
CKM/CKB Antibody (Clone#AFOC-3) (Boster Biological Technology, Wuhan, China. Catalog#BM5643)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐稀释比
Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-200 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
ImmunoPrecipitation (IP): | 1:50 |
Flow Cytometry (FCM): | 1:50 |