产品名称
Human FGF1 ELISA Kit
指标别名
AFGF; ECGF; ECGF beta; ECGFA; ECGFB; FGF alpha; FGF1; FGFA; GLIO703; HBGF 1; HBGF1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
fibroblast growth factor 1 (acidic)
文献引用格式
Human FGF1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0339)
背景资料
Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0339-CAP | 96-well plate precoated with anti- human FGF1 antibody | 1 |
| EK0339-ST | Lyophilized recombinant human FGF1 standard | 10ng/tube×2 |
| EK0339-DA | Biotinylated anti- human FGF1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 59 | 282 | 984 | 58 | 270 | 1073 |
| Standard deviation | 3.3 | 15.22 | 76.75 | 3.82 | 18.9 | 98.71 |
| CV(%) | 5.6 | 5.4 | 7.8 | 6.6 | 7 | 9.2 |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.032 | 0.157 | 0.231 | 0.351 | 0.536 | 0.962 | 1.478 | 1.981 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)