产品名称
Mouse FGF1 ELISA Kit
指标别名
AFGF; ECGF; ECGF beta; ECGFA; ECGFB; FGF alpha; FGF1; FGFA; GLIO703; HBGF 1; HBGF1
免疫原
Expression system for standard: E.coli; Immunogen sequence: F16-D155
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Fibroblast growth factor 1
文献引用格式
Mouse FGF1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1172)
背景资料
Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1172-CAP | 96-well plate precoated with anti- mouse FGF1 antibody | 1 |
| EK1172-ST | Lyophilized recombinant mouse FGF1 standard | 10ng/tube×2 |
| EK1172-DA | Biotinylated anti- mouse FGF1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 67 | 295 | 874 | 73 | 276 | 798 |
| Standard deviation | 4.22 | 12.98 | 54.18 | 4.74 | 14.07 | 61.44 |
| CV(%) | 6.3% | 4.4% | 6.2% | 6.5% | 5.1% | 7.7% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.025 | 0.215 | 0.321 | 0.468 | 0.752 | 1.181 | 1.777 | 2.615 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)