指标别名
AFGF; ECGF; ECGF beta; ECGFA; ECGFB; FGF alpha; FGF1; FGFA; GLIO703; HBGF 1; HBGF1
免疫原
Expression system for standard: E.coli; Immunogen sequence: F16-D155
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
fibroblast growth factor 1 (acidic)
文献引用格式
Rat FGF1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1173)
背景资料
Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization.
试剂盒内容
| Catalog number | Description | Quantity |
| EK1173-CAP | 96-well plate precoated with anti- rat FGF1 antibody | 1 |
| EK1173-ST | Lyophilized recombinant rat FGF1 standard | 10ng/tube×2 |
| EK1173-DA | Biotinylated anti- rat FGF1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 101 | 288 | 887 | 96 | 312 | 856 |
| Standard deviation | 7.97 | 19.58 | 37.25 | 9.12 | 26.2 | 37.66 |
| CV(%) | 7.9% | 6.8% | 4.2% | 9.5% | 8.4% | 4.4% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.065 | 0.244 | 0.355 | 0.508 | 0.739 | 1.093 | 1.586 | 2.316 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)