产品名称
Human MCP-2/CCL8 ELISA Kit
指标别名
CCL8; MCP2; SCYA10; SCYA8; C-C motif chemokine 8; HC14; Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; MCP-2; Small-inducible cytokine A8; MCP-2(6-76)
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名全称
chemokine (C-C motif) ligand 8
文献引用格式
Human MCP-2/CCL8 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0442)
背景资料
Chemokine(C-C motif) ligand 8, also known as monocyte chemoattractant protein 2(MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2(MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0442-CAP | 96-well plate precoated with anti- human CCL8 antibody | 1 |
| EK0442-ST | Lyophilized recombinant human CCL8 standard | 10ng/tubex2 |
| EK0442-DA | Biotinylated anti- human CCL8 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 35 | 152 | 451 | 36 | 154 | 486 |
| Standard deviation | 2.76 | 9.27 | 19.84 | 3.13 | 11.24 | 27.7 |
| CV(%) | 7.9 | 6.1 | 4.4 | 8.7 | 7.3 | 5.7 |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.011 | 0.086 | 0.177 | 0.375 | 0.683 | 1.197 | 1.661 | 2.024 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)