产品名称
Mouse MCP-2/CCL8 ELISA Kit
指标别名
CCL8; MCP2; SCYA10; SCYA8; C-C motif chemokine 8; HC14; Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; MCP-2; Small-inducible cytokine A8; MCP-2(6-76)
免疫原
Expression system for standard: E.coli; Immunogen sequence: E20-P97
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
C-C motif chemokine 8
文献引用格式
Mouse MCP-2/CCL8 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1137)
背景资料
Chemokine (C-C motif) ligand 8, also known as monocyte chemoattractant protein 2 (MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2 (MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1137-CAP | 96-well plate precoated with anti- mouse CCL8 antibody | 1 |
| EK1137-ST | Lyophilized recombinant mouse CCL8 standard | 1ng/tube×2 |
| EK1137-DA | Biotinylated anti- mouse CCL8 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 37 | 190 | 475 | 33 | 180 | 452 |
| Standard deviation | 2.66 | 9.12 | 28.5 | 2.73 | 9 | 27.12 |
| CV(%) | 7.2% | 4.8% | 6% | 8.3% | 5% | 6% |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.055 | 0.137 | 0.182 | 0.295 | 0.496 | 0.897 | 1.563 | 2.225 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)