产品名称
Human TIMP2 ELISA Kit
指标别名
CSC 21K; Metalloproteinase inhibitor 2; TIMP 2; TIMP2; TIMP-2
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
TIMP metallopeptidase inhibitor 2
文献引用格式
Human TIMP2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0522)
背景资料
TIMP2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs(kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0522-CAP | 96-well plate precoated with anti- human TIMP-2 antibody | 1 |
EK0522-ST | Lyophilized recombinant human TIMP-2 standard | 10ng/tubex2 |
EK0522-DA | Biotinylated anti- human TIMP-2 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 249 | 1786 | 4399 | 239 | 1654 | 4356 |
Standard deviation | 15.93 | 94.65 | 325.52 | 16.73 | 112.47 | 361.54 |
CV(%) | 6.4 | 5.3 | 7.4 | 7 | 6.8 | 8.3 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.005 | 0.109 | 0.198 | 0.418 | 0.787 | 1.397 | 2.109 | 2.513 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Saliva