指标别名
CSC 21K; Metalloproteinase inhibitor 2; TIMP 2; TIMP2; TIMP-2
免疫原
Expression system for standard: NS0; Immunogen sequence: C27-P220
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
TIMP metallopeptidase inhibitor 2
文献引用格式
Rat TIMP2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1302)
背景资料
TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.
试剂盒内容
Catalog number | Description | Quantity |
EK1302-CAP | 96-well plate precoated with anti- rat TIMP-2 antibody | 1 |
EK1302-ST | Lyophilized recombinant rat TIMP-2 standard | 10ng/tube×2 |
EK1302-DA | Biotinylated anti- rat TIMP-2 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 240 | 1342 | 4533 | 227 | 1463 | 4769 |
Standard deviation | 10.56 | 68.44 | 339.97 | 10.21 | 95.09 | 443.51 |
CV(%) | 4.4% | 5.1% | 7.5% | 4.5% | 6.5% | 9.3% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 313 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.080 | 0.123 | 0.177 | 0.233 | 0.386 | 0.917 | 1.401 | 1.938 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)